Journal: International Journal of Molecular Sciences

Article Title: Proinflammatory Cytokine Preconditioning Enhances the Therapeutic Potency of Different Types of MSCs in Inflammation

doi: 10.3390/ijms27094090

Figure Lengend Snippet: Cytomix increased the expression of immunomodulatory markers on BM-MSCs, iMSCs WT and iMSCs B2M KO. The gating strategy is shown in . ( A ) Histogram charts of the expression of the immunomodulatory markers on BM-MSCs, iMSCs WT and iMSCs B2M KO. ( B – H ) Cytomix significantly increased the expression levels of HLA-ABC on BM-MSCs and iMSCs WT, HLA-DR on BM-MSCs, CD54 on all types of MSCs, CD200 on iMSCs WT, CD119 and CD120b on BM-MSCs, and CD274 on iMSCs WT and iMSCs B2M KO. Representative results of three independent experiments are shown. Results are shown as mean ± SD ( n = 3 in each group) and analysed by two-way ANOVA with Šídák’s multiple comparisons test or Tukey’s multiple comparisons test. */**/***/**** represent comparisons of naïve vs. Cytomix in each type of MSC; $/$$$/$$$$ represent comparisons of BM-MSCs vs. iMSCs WT vs. iMSCs B2M KO in the naïve group; ##/###/#### represent comparisons of BM-MSCs vs. iMSCs WT vs. iMSCs B2M KO in the Cytomix group with significance levels of p < 0.05/0.01/0.001/0.0001, respectively. Nd represents not detectable.

Article Snippet: The cells were centrifuged at 300× g for 5 min and resuspended in 100 μL FACS buffer containing anti-human HLA-ABC-VioGreen, HLA-DR-VioBlue, CD54-APC, CD274-PE-Vio ® 615, CD119-FITC (Cat# 130-120-436, 130-111-794, 130-121-342, 130-122-811, 130-099-931; Miltenyi Biotec, Bergisch Gladbach, Germany), CD120b-PE and CD200-PE-CY7 antibodies (Cat# 358403, 399805; BioLegend, San Diego, CA, USA).

Techniques: Expressing

Journal: bioRxiv

Article Title: Single-cell Analysis of Paired FFPE Placentas Reveals Trophoblast Reprogramming and Immune Dysregulation in Chronic Villitis of Unknown Etiology

doi: 10.64898/2026.02.28.708431

Figure Lengend Snippet: (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

Article Snippet: For multiplex immunohistochemical staining, primary antibodies are against human CK7 (17513, Proteintech), and HLA-A/B (15240-1-AP, Proteintech), the Four Detect Kit for Rabbit Primary Antibody kit (PK10033, Proteintech) used for multiple staining.

Techniques: Expressing, Control, Multiplex Assay, Immunohistochemistry

Journal: bioRxiv

Article Title: Single-cell Analysis of Paired FFPE Placentas Reveals Trophoblast Reprogramming and Immune Dysregulation in Chronic Villitis of Unknown Etiology

doi: 10.64898/2026.02.28.708431

Figure Lengend Snippet: (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

Article Snippet: For multiplex immunohistochemical staining, primary antibodies are against human CK7 (17513, Proteintech), and HLA-A/B (15240-1-AP, Proteintech), the Four Detect Kit for Rabbit Primary Antibody kit (PK10033, Proteintech) used for multiple staining.

Techniques: Expressing, Control, Multiplex Assay, Immunohistochemistry